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SUBCELLULAR DISTRIBUTION OF AROMATIC AMINO ACID TRANSAMINASES IN RAT BRAIN
Author(s) -
Semba T.,
Civen M.
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb03351.x
Subject(s) - transaminase , aspartate transaminase , biochemistry , elevated transaminases , biology , alanine transaminase , microsome , tryptophan , enzyme , chemistry , medicine , amino acid , endocrinology , alkaline phosphatase
Abstract— The subcellular distributions of tyrosine transaminase, DOPA transaminase, tryptophan transaminase and 5‐hydroxytryptophan (5‐HTP) transaminase were studied in rat brain.1 For all of these transaminases 60‐81 per cent of the total activities were found in the crude mitochondrial fraction. Tyrosine transaminase was the most active enzyme.2 Tyrosine transaminase and DOPA transaminase had very similar distributions in all fractions, but the distribution of tryptophan transaminase and 5‐HTP transaminase differed in the microsomal (Mic) and synaptic vesicle (M 2 ) fractions. Only 5‐HTP transaminase was highly concentrated in the M 2 fraction.3 DOPA transaminase was inhibited by dopamine and 5‐HT, but these compounds had no effect on 5‐HTP transaminase. Both enzymes were completely inhibited by m ‐hydroxybenzoyloxyamine.