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TOXIC EFFECTS OF OXYGEN ON CEREBRAL METABOLISM
Author(s) -
Williams Carolyn D.,
Haugaard N.
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb03341.x
Subject(s) - oxygen , metabolism , glutathione , substrate (aquarium) , glycolysis , oxidative phosphorylation , dithiothreitol , carbohydrate metabolism , biochemistry , chemistry , toxicity , oxygen toxicity , biology , enzyme , organic chemistry , ecology
— The effect of 100% O 2 at one atmosphere on carbohydrate metabolism in cell‐free homogenates of rat brain was studied under different experimental conditions. The principal findings were the following:1 Compared to 10% O 2 ‐90% N 2 , oxygen at one atmosphere inhibited metabolism of α‐oxoglutarate and depressed the net synthesis of ATP. With glucose as substrate, accumulation of ATP was also markedly inhibited but substrate utilization was only slightly affected.2 Glycolysis in brain was relatively resistant to oxygen toxicity, except in the presence of added Cu 2+ .3 With α‐oxoglutarate as the substrate, inhibition of the formation of ATP occurred earlier than inhibition of substrate utilization, indicating the particular sensitivity of oxidative phosphorylation to inactivation by oxygen in vitro.4 Cu 2+ and Fe 2+ accentuated oxygen toxicity but appeared to act by different mechanisms. Co 2+ exerted a protective effect.5 The sulphydryl compounds, dithiothreitol and reduced glutathione, strongly diminished the toxic effect of oxygen.