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THE EFFECT OF N ‐METHYL‐4‐PIPERIDYL‐DIPHENYL GLYCOLATE ON THE INCORPORATION OF 32 P INTO PHOSPHOLIPIDS FROM RAT BRAIN CORTEX SLICES AND ITS SUBCELLULAR LOCALIZATION
Author(s) -
Heilbronn E.,
Widlund L.
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb02257.x
Subject(s) - phosphatidic acid , oxotremorine , chemistry , phosphatidylinositol , phosphatidylethanolamine , acetylcholine , cortex (anatomy) , stimulation , endocrinology , atropine , biochemistry , medicine , muscarinic acetylcholine receptor , phospholipid , phosphatidylcholine , biology , membrane , receptor , kinase , neuroscience
— N ‐Methy‐4‐piperidy1‐diphenyl glycolate ( N ‐methy1‐4‐piperidy1 benzilate), an anticholinergic drug, was shown to stimulate 32 P‐incorporation into total phospholipids of rat brain cortex slices. Analysis of the total phospholipids showed stimulation in phosphatidic acid, phosphatidylinositol and phosphatidylethanolamine. Stimulated 32 P‐incorpora‐tion was accompanied by a decrease in the Q o 2 (μ1 O 2 /mg dry tissue) value. The effects of the drug were compared with those of some other CNS‐active drugs known to interfere with the ACh content of brain; tremorine, oxotremorine and atropine; and in the presence of eserine, with that of the neurotransmitter acetylcholine. Increase of the outer K+‐concentration resulted in increased Q o 2 and 32 P‐incorporation into the slices. The effect of the glycolate and perhaps that of atropine tended to increase in this medium. Subcellular fractionation of slices showed that the glycolate stimulated 32 P‐incorporation occurred mainly in the nerve end fraction. The total amount of the individual phospholipids was not changed, but the specific activity had significantly increased in phosphatidic acid and phosphatidylinositol. The possibility that glycolate‐induced stimulated 32 P‐incorporation into nerve end phospholipids is due to increased glycolysis is discussed.