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THE DISC ELECTROPHORETIC SEPARATION OF PROTEINS FROM VARIOUS PARTS OF THE GUINEA PIG BRAIN 1
Author(s) -
Ewart Davies W.
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb02216.x
Subject(s) - guinea pig , electrophoresis , inferior colliculus , cerebellar cortex , biology , myelin , cortex (anatomy) , nucleus , biochemistry , chemistry , cerebellum , microbiology and biotechnology , neuroscience , central nervous system , endocrinology
—Disc electrophoresis was used to study the saline‐soluble and detergent‐soluble proteins of various parts of the auditory pathway in the guinea pig brain. The five areas studied were the cochlear nucleus, olivary complex, inferior colliculus, medial geniculate and the auditory cortex. A study was also made of the proteins extracted from subcellular fractions obtained from guinea pig cerebral cortex. The electrophoretic patterns showed small but significant differences in the five areas of the brain, both in the fast‐running acidic proteins and in the very slow‐moving proteins. The higher levels of the auditory pathway, to which the more complex information processing and storage is normally attributed, showed more complex electrophoretic patterns than the lower areas. Gross differences also occurred in the patterns of both the saline‐soluble and T‐X‐100‐soluble proteins of the subcellular fractions obtained by gradient density centrifugation. The simplest patterns were obtained from the myelin‐rich fraction and the mitochondrial fraction whilst the most complex patterns were given by the proteins of the nerve ending fraction and the cell soluble fraction.