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GLYCOPROTEINS OF SUBMAXILLARY SALIVA OF THE CAT: DIFFERENCES IN COMPOSITION PRODUCED BY SYMPATHETIC AND PARASYMPATHETIC NERVE STIMULATION 1
Author(s) -
Dische Z,
Kahn N,
Rothschild C,
Danilchenko A,
Licking J,
Wang S. C
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb00544.x
Subject(s) - saliva , stimulation , medicine , endocrinology , chemistry , submaxillary gland , neuraminic acid , fucose , glycoprotein , biochemistry
— Saliva samples were obtained from the cannulated submaxillary ducts of the cat during stimulation of the peripheral cut end(s) of (1) the cervical sympathetic nerve, (2) the chordalingual (parasympathetic) nerve and (3) both nerves at the same time. In nine experiments the ratios of neuraminic acid to fucose and to hexosamine were consistently 2·5–4 times higher in saliva evoked by sympathetic nerve stimulation than in that produced by parasympathetic stimulation. This was not attributable to differences in the rate of synthesis of the carbohydrate of the glycoproteins or in salivary flow rate. The presence of glycolipids and blood glycoproteins was excluded. Saliva produced by stimulation of the sympathetic and parasympathetic nerves each showed a single, but different, peak after ultracentrifugation in 0·1 m ‐NaCl with 0·01 m ‐phosphate buffer (pH 7·4). The S 20, w of the former was 6·5 and of the latter, 39. Both peaks were demonstrable in saliva produced when both nerves were stimulated at the same time.