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A LIGHT‐SCATTERING TECHNIQUE FOR THE STUDY OF THE PERMEABILITY OF RAT BRAIN SYNAPTOSOMES IN VITRO
Author(s) -
Keen P,
White T. D
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb00535.x
Subject(s) - synaptosome , chemistry , formamide , tonicity , permeability (electromagnetism) , glycerol , chromatography , ammonium chloride , nuclear chemistry , biophysics , membrane , biochemistry , organic chemistry , biology
— (1) Swelling of synaptosomes was measured spectrophotometrically by recording changes in extinction at 520 nm. (2) Synaptosomes behaved as osmometers in NaCl solutions. When the tonicity of the medium was changed, synaptosome volume changed in accordance with Boyle and van't Hoff's Law. These changes were reversed on restoring the tonicity of the medium. (3) The rate at which a solute entered the synaptosome was determined from the rate of swelling in the presence of that solute. Permeability of synaptosomes to non‐electrolytes was in the order glucose ≪ glycerol < thiourea = formamide < propylene glycol = dimethylsulphoxide. (4) Synaptosomes were freely permeable to ammonium and acetate ions and impermeable to Ca 2+ , Mg 2+ , PO 4 2− , SO 4 2− and oxalate ions.