Premium
METABOLISM OF HIGHLY BASIC PROTEINS OF RAT BRAIN DURING POSTNATAL DEVELOPMENT
Author(s) -
Gaitonde M. K,
Martenson R. E
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb00534.x
Subject(s) - major basic protein , myelin basic protein , proteolipid protein 1 , metabolism , biochemistry , biology , protein metabolism , blood proteins , myelin , period (music) , chemistry , endocrinology , central nervous system , immunology , eosinophil , asthma , physics , acoustics
— (1) The encephalitogenic basic protein obtained from adult rat brain by treatment with 0·03 N‐HCl was demonstrable in the brain on the 10th day after birth. It showed a marked increase in quantity during the phase of active myelination. (2) The proteins extracted under similar conditions from 5‐day old rat brain contained several highly basic proteins other than the encephalitogenic basic protein. These basic proteins, which were electrophoretically similar to highly basic proteins extracted similarly from adult rat liver, are histones. (3) For metabolic studies the entire group of highly basic proteins in the acid extract was obtained after one‐step adsorption of other proteins on DEAE‐cellulose equilibrated at pH 9·8 (4) After injection of [ 14 C]lysine the fractions containing highly basic proteins, water soluble non‐basic proteins and other tissue proteins of the brain showed higher relative specific radioactivities during the period 1–10 days after birth than during later stages of postnatal development. The fraction containing proteolipid protein, another myelin protein, showed a low relative specific radioactivity throughout the whole period of postnatal development. The relative specific radioactivity of proteolipid protein was somewhat higher in young than in adult rat brain.