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BINDING OF d ‐[ DIMETHYL 14 C]TUBOCURARINE, ACETYLCHOLINESTERASE AND PROTEOLIPIDS IN SUBCELLULAR MEMBRANES OF CEREBRAL CORTEX OF THE DEVELOPING RAT 1
Author(s) -
Gömez C. J,
Pasquini Juana M,
Soto E. F,
Robertis E. De
Publication year - 1970
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1970.tb00515.x
Subject(s) - acetylcholinesterase , cerebral cortex , cortex (anatomy) , cholinergic , acetylcholine , mole , biochemistry , electron microscope , membrane , biophysics , synaptic membrane , chemistry , biology , enzyme , endocrinology , neuroscience , physics , optics
— The cerebral cortex of rats at postnatal ages of 5,15,30 and 50 days was homogenized and fractionated to separate the crude mitochondrial fraction. This fraction was osmotically shocked and the Mi fraction and subfractions were separated. The variations with age in the morphological composition of subfractions M 1 0·8, Mi 1·3 and M 1 p were studied under the electron microscope. Because of the changes observed in the various fractions the need for such type of control is stressed. The changes in total protein and proteolipid‐protein of the fractions at different ages, as well as the acetylcholinesterase activity and the binding of d ‐[ dimethyl −14 C]tubocurarine were studied. The results obtained were interpreted on the basis of the important morphogenetic changes that the cerebral cortex undergoes postnatally. The progressive and parallel increase in acetylcholinesterase and in the binding of d ‐[ dimethyl ‐ 14 C]tubocurarine to proteolipids of fraction Mi 1·3, suggest a close relationship between these two events and the development of the cholinergic synapses.