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PROTEIN METABOLISM IN GOLDFISH BRAIN 1
Author(s) -
Lim R.,
Agranoff B. W.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb10384.x
Subject(s) - leucine , in vivo , metabolism , tritium , protein metabolism , protein turnover , amino acid , isotope , biochemistry , chemistry , intraperitoneal injection , biology , protein biosynthesis , endocrinology , medicine , physics , microbiology and biotechnology , quantum mechanics , nuclear physics
— Protein metabolism of goldfish brain was studied in vivo by means of intraperitoneal or intracranial injections of [ 3 H]leucine and compared with concomitant studies in the mouse. Heterogeneity of turnover values was observed. Long turnover times were seen relative to other organs examined. The free amino acid pools of goldfish brain were determined, and the fate of tritium from labelled leucine was followed at various times after injection. Following ‘chasing’ with large amounts of unlabelled leucine or protein inhibitors shortly after isotope injection, further incorporation was arrested, but examination of the labelled protein over a period of 2 weeks indicated a slow decay, similar to that seen without ‘chasing’. Possible use of ‘pulse‐chase’ experiments in vivo in animals is discussed in relation to behavioural studies.