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HISTOCHEMICAL DEMONSTRATION OF ACID RIBONUCLEASE IN THE CENTRAL NERVOUS SYSTEM
Author(s) -
Ghosh Swati,
Ghosh J. J.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb10373.x
Subject(s) - incubation , acid phosphatase , staining , ribonuclease , cytoplasm , biochemistry , incubation period , enzyme , nervous tissue , subcellular localization , alkaline phosphatase , chemistry , central nervous system , biology , microbiology and biotechnology , rna , neuroscience , gene , genetics
— The staining pattern of acid ribonuclease activity in the nervous tissues of rats has been studied with different fixatives and incubation media. Certain modifications in the method adopted (V orbrodt , 1961) produced a definite improvement in the localization as well as distinct subcellular distribution at the pHs studied. The modifications introduced consisted of preincubation of tissue sections with prostatic acid phosphatase, omission of acid phosphatase from the standard incubation medium and its addition at a later stage of incubation. This avoids much of the diffused cytoplasmic and filamentous background staining at pH 5.2–5.8, eliminates the irregular nuclear staining at pH 4.5 and replaces the strong nuclear positivity at pH 5.2 by a fine granular dispersion. A study of the enzyme activity of subcellular fractions at the different pHs used was performed to confirm the histochemical localization.