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UPTAKE OF CALCIUM BY SUBCELLULAR FRACTIONS ISOLATED FROM OUABAIN‐TREATED CEREBRAL TISSUES
Author(s) -
Stahl W. L.,
Swanson P. D.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb10354.x
Subject(s) - ouabain , calcium , homogenization (climate) , chemistry , cerebral cortex , mole , biochemistry , biophysics , endocrinology , medicine , sodium , biology , biodiversity , ecology , organic chemistry
— Slices of cerebral cortex were incubated in medium containing 0·75 or 2·8 mM 45 CaCl 2 , in the presence or absence of 0·01–0·1 m m ‐ouabain. Ouabain induced accumulation of calcium by slices to a maximum of 4 μmoles/g of tissue/hr (0·75 m m ‐CaCl 2 in the medium) and to 8 μmoles/g of tissue/hr (2·8 m m ‐CaCl 2 in the medium). Accumulation of Ca 2+ occurred more slowly than loss of K + from the slices and more closely resembled the pattern of Na + uptake. Mitochondrial fractions isolated from ouabain‐treated slices contained significantly more calcium than controls. Inclusion of EDTA in the homogenization medium resulted in decreased amounts of particulate‐bound calcium. The effect of ouabain on accumulation of calcium is discussed with regard to possible relationships to processes of active and passive transport.