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AN IN VITRO SYSTEM FOR THE STUDY OF MYELIN SYNTHESIS 1
Author(s) -
Smith Marion E.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb10345.x
Subject(s) - myelin , cerebroside , in vitro , lecithin , biochemistry , spinal cord , myelin sheath , chemistry , myelin basic protein , biology , central nervous system , endocrinology , neuroscience
— A system for the study of short‐term myelin synthesis in spinal cord tissue in vitro with [U‐ 14 C]glucose as a lipid and protein precursor is described. The rates of lipid and protein incorporation into myelin are age‐dependent, but do not appear to behave similarly. Uptake of labelled carbon is most rapid in monophosphoinositide and lecithin, and slowest in cerebroside sulphate. Myelin protein seems to retain more metabolic activity than does the lipid. These findings are interpreted as support for the unit membrane hypothesis for myelin rather than the subunit model.