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EFFECT OF IONS ON STIMULUS‐INDUCED RELEASE OF AMINO ACIDS FROM MAMMALIAN BRAIN SLICES
Author(s) -
Katz R. I.,
Chase T. N.,
Kopin I. J.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb08986.x
Subject(s) - amino acid , stimulation , potassium , calcium , depolarization , efflux , chemistry , aminoisobutyric acid , biochemistry , stimulus (psychology) , biophysics , medicine , biology , endocrinology , psychology , organic chemistry , psychotherapist
— Slices of mammalian brain accumulate amino acids contained in physiological medium. When such tissues were subjected to mild electrical stimulation of short duraation capable of depolarizing neural membranes, there occurred a striking increase in the efflux of exogenous amino acids. The effects on representative acidic, neutral, and basic amino acids were similar. Elevated levels of potassium chloride evoked release of amino acids comparable to electrical stimulation. Electrically stimulated release of [ 3 H]γ‐aminobutyric acid was not inhibited by the presence of reduced concentrations of calcium ions. Although amino acids are actively accumulated by liver and kidney slices, electrical stimulation of these tissues failed to release these compounds. Stimulation‐induced release was significantly diminished by the presence of small amounts of lithium in the perfusing medium.