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THE EFFECT OF PHOSPHOLIPASES ON THE UPTAKE OF ATROPINE AND ACETYLCHOLINE BY SLICES OF MOUSE BRAIN CORTEX
Author(s) -
Heilbronn E.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb06862.x
Subject(s) - atropine , inulin , chemistry , ouabain , acetylcholine , phospholipase , potassium , medicine , endocrinology , physostigmine , sodium , biochemistry , enzyme , biology , organic chemistry
— The uptake of [ 3 H]atropine, [ 3 H]acetylcholine and [ 14 C]inulin in mouse brain cortex slices was studied in slices treated with phospholipases A or C. In control experiments the slices took up atropine and acetylcholine against a concentration gradient, indicating active uptake. This uptake was decreased by ouabain, by anaerobic conditions and by an increase in the potassium ion concentration. The phospholipases were found to decrease the uptake of atropine and particularly that of acetylcholine in the slices. The uptake of labelled inulin in enzyme‐treated slices, as compared to untreated slices, was not decreased, indicating no change in the inulin space. The effect of the phospholipases was time dependent and, up to a certainlimit, concentration dependent. The effect of ouabain in decreasing the uptake of atropine was not eliminated by the enzyme treatment. The effect of anaerobic conditions in decreasing the uptake was weak after treatment with phospholipases. The effect of higher concentrations of potassium ions was abolished by treatment with phospholipase A. The results emphasize the importance of phospholipids as substances controlling structural order in membranes and suggest their participation in active transport.