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DNA SYNTHESIS IN THE GLIAL CELLS OF SCRAPIE‐AFFECTED MOUSE BRAIN
Author(s) -
Kimberlin R. H.,
Anger H. S.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb06853.x
Subject(s) - scrapie , biology , incubation period , thymidine , dna , sagittal plane , incubation , pathogenesis , pathology , anatomy , immunology , medicine , genetics , disease , biochemistry , prion protein
— Radioautographic techniques have been used to study the incorporation of [ 3 H]‐thymidine into the DNA of glial cells in normal and scrapie‐affected mouse brain. Some differences were seen in the morphological distribution and also in the size distribution of labelled nuclei in sagittal sections of normal and affected brain. Counts of the total number of labelled nuclei per sagittal section showed a progressive decline from 50 to 15 per section in normal brain over the 4‐month experimental period. However, in scrapie‐affected brain the number increased markedly to 120 per section 5‐6 weeks after inoculation and stayed at this level for the remaining 9‐10 weeks of the incubation period. The significance of these results in relation to the pathogenesis of scrapie is discussed.