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OCCURRENCE OF A SEROTONIN SULPHOTRANSFERASE IN THE BRAIN
Author(s) -
Hidaka H.,
Nagatsu T.,
Yagi K.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb06457.x
Subject(s) - serotonin , cerebellum , specific activity , fractionation , enzyme , biochemistry , chromatography , ammonium , chemistry , biology , endocrinology , receptor , organic chemistry
— —An enzyme catalysing the transfer of sulphate from 3′‐phosphoadenylsulphate to serotonin was purified from rabbit brain. The purification procedure involved ammonium sulphate fractionation of the 200,000 g supernatant of rabbit brain homogenate, treatment with alumina Cγ, and chromatography on DEAE‐cellulose. The enzyme was purified 67‐fold from the 200,000 g supernatant of the brain homogenate. The intracranial distribution of the sulphotransferase was investigated and the cerebellum found to have rather high activity. The sulphotransferase activities of rabbit, dog, rat and bovine brains were compared; rabbit brain had the highest activity, followed by dog, rat and bovine brain.