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PROTEIN TURNOVER IN CELL‐ENRICHED FRACTIONS FROM RABBIT BRAIN 1
Author(s) -
Blomstrand C.,
Hamberger A.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb05992.x
Subject(s) - spinal cord , cell , leucine , biology , central nervous system , fraction (chemistry) , chemistry , biochemistry , endocrinology , amino acid , chromatography , neuroscience
— A modification of the available methods was used for preparation of nerve and glia cell‐enriched fractions from rabbit brain and spinal cord. The rate of incorporation of tritiated leucine and the turnover rates of protein during 10 days was studied in the bulkprepared cell fractions. The rate of incorporation into the nerve cell fraction was approximately three times greater than in the glia fraction. The nerve cells had one rapid and one slow phase in decline of radioactivity, while the glial cells were characterized by a more uniform decline. The soluble radioactivity was followed in whole tissue of brain and spinal cord and certain differences between the two were observed.