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SUBCELLULAR DISTRIBUTION AND CHEMICAL NATURE OF THE RECEPTOR FOR 5‐HYDROXYTRYPTAMINE IN THE CENTRAL NERVOUS SYSTEM 1
Author(s) -
Fiszer Sara,
Robertis E.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb05967.x
Subject(s) - tryptamine , chemistry , membrane , reserpine , myelin , chromatography , cortex (anatomy) , biochemistry , central nervous system , biology , endocrinology , neuroscience
— In vitro binding experiments with 5‐hydroxy[ 14 C]tryptamine (3.3 × 10 −6 M) were carried out on subcellular fractions of the cat brain. The highest specific activity was observed in some fractions of nerve‐ending membranes isolated from the hypothalamus, basal ganglia, and gray areas of the mesencephalon. The specificity of this high affinity binding was demonstrated by competition with reserpine, butanolamide of lysergic acid, and desmethylimipramine. With butanol‐water extraction the [ 14 C]5‐HT was found in the butanol while the gangliosides were separated in the water phase. Several experiments with thin layer and column chromatography suggest that in the organic phase the [ 14 C]5‐HT is not bound to the lipids but to a special proteolipid. This proteolipid is different from that found in myelin and has similar chromatographic properties to that previously observed in the proteolipid which binds d‐[ 14 C]tubocurarine in nerve‐ending membranes of the cerebral cortex.