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ISOLATION AND CHARACTERIZATION OF GLYCOSAMINOGLYCANS IN BRAIN OF DIFFERENT SPECIES
Author(s) -
Singh M.,
Chandrasekaran E. V.,
Cherian R.,
Bachhawat B. K.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb05961.x
Subject(s) - glycosaminoglycan , hyaluronic acid , hyaluronidase , uronic acid , chondroitin , sephadex , galactosamine , biochemistry , glucosamine , chemistry , pyridinium , chondroitin sulphate , chromatography , hexosamines , biology , enzyme , polysaccharide , anatomy , medicinal chemistry
— The uronic acid‐containing glycosaminoglycans present in the brains of rat, monkey, chicken, sheep and rabbit were isolated into various fractions by combining the cetyl pyridinium procedure and DEAE‐Sephadex column chromatography. The analyses of the fractions show that hyaluronic acid, chondroitin‐4‐sulphate, chondroitin‐6‐sulphate, heparan sulphate and a testicular hyaluronidase‐resistant galactosamine‐containing GAG are present in the brain of all the species studied. Hyaluronic acid is the major GAG (33–41 per cent). Chondroitin‐4‐sulphate (19–35 per cent), and heparan sulphate (11–19 per cent), are the next prominent GAGs, in all the species except chicken. The results indicate the similarity in the pattern of GAGs in the brain of all the species.