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INCORPORATION OF 32 P IN VITRO INTO TRIPHOSPHOINOSITIDE AND RELATED LIPIDS OF RAT SUPERIOR CERVICAL GANGLIA AND VAGUS NERVES
Author(s) -
Yagihara Y.,
Salway J. G.,
Hawthorne J. N.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb05958.x
Subject(s) - vagus nerve , inositol , sciatic nerve , phosphatidylinositol , in vitro , incubation , chemistry , cervical ganglia , endocrinology , calcium , medicine , phosphate , anatomy , biochemistry , biology , receptor , stimulation , kinase
— Rat sympathetic ganglia, vagus nerve and sciatic nerve were each incubated with inorganic 32 P for various lengths of time and the resultant labelling of their inositol lipids was measured. At all times up to 3 hr phosphatidylinositol was the most highly labelled lipid of ganglia, while triphosphoinositide was the most active lipid of vagus and sciatic nerves. Removal of calcium ions from the incubation media had no significant effect on the incorporation of phosphate into any of the inositol lipids of sympathetic ganglia.