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THE MEASUREMENT OF TRIGLYCERIDE IN BRAIN AND THE METABOLISM OF BRAIN TRIGLYCERIDE IN VITRO
Author(s) -
Rowe C. E.
Publication year - 1969
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1969.tb05938.x
Subject(s) - triglyceride , hydrolysis , chemistry , lipase , in vitro , lipoprotein lipase , biochemistry , cortex (anatomy) , metabolism , brain cortex , cerebellum , endocrinology , medicine , chromatography , biology , enzyme , cholesterol , neuroscience
Abstract—1 Triglyceride has been isolated from brain by thin‐layer chromatography and determined by absorption of the carbonyl group at 1740 cm −1 . The means of yields from whole mouse brain, whole rat brain, rat brain grey matter, rat brain stem, and incubated slices of rat brain cortex were 0.15–0.17 μmole/g tissue. 2 The distribution of fatty esters varied from preparation to preparation. Palmitate, stearate and oleate usually occurred in greatest amounts. Hydrolysis of a preparation of triglyceride from whole rat brain with pancreatic lipase indicated that palmitate was equally distributed between the α and β esters. 3 [1‐ 14 C]Acetate was rapidly incorporated into triglyceride of slices of incubated rat brain cortex. When the resulting triglyceride was hydrolysed with pancreatic lipase the distribution of radioactivity amongst the hydrolysis products was consistent with both the α and β esters of the triglyceride having been radioactively labelled.