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COMPARISON OF 5‐HYDROXYINDOLES IN VARIOUS REGIONS OF DOG BRAIN AND CEREBROSPINAL FLUID
Author(s) -
Eccleston D.,
Ashcroft G. W.,
Moir A. T. B.,
ParkerRhodes A.,
Lutz W.,
O'Mahoney D. P.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb11637.x
Subject(s) - tryptophan , cerebrospinal fluid , serotonin , tryptophan hydroxylase , endocrinology , 5 hydroxytryptophan , medicine , chemistry , biology , biochemistry , amino acid , serotonergic , receptor
— —Normal values for the concentration of 5‐HT, 5‐HIAA and tryptophan are established in various regions of the dog brain. After administration of tryptophan by intravenous injection the rise and fall of 5‐HT and 5‐HIAA were estimated at 1, 2 and 4 hr. Best fit quadratic regression curves obtained by computer programme were fitted to the data. Similar tryptophan doses were given to dogs and the 5‐HIAA concentration estimated in the cisternal CSF. Quadratic regression curves fitted to these values show that the concentration of 5‐HIAA in CSF reflects the changes of 5‐HIAA in the brain and in particular in the brain stem. a‐Methyl dopa pretreatment blocked the rise of 5‐hydroxyindoles in brain and CSF and appeared to inhibit tryptophan hydroxylase as well as decarboxylase.