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METABOLISM OF SPECIFICALLY LABELLED GLUCOSE BY EXPLANTS OF NEWBORN MOUSE CEREBELLUM *
Author(s) -
Hoskin F. C. G.,
Allerand C. Dominique
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb11629.x
Subject(s) - neurochemical , cerebellum , pentose phosphate pathway , myelin , metabolism , biochemistry , biology , carbohydrate metabolism , tissue culture , chemistry , glycolysis , endocrinology , central nervous system , in vitro
— Newborn mouse cerebellum developing in culture metabolizes specifically labelled glucose to labelled carbon dioxide at a high rate and in a manner indicating virtually no participation of the pentose phosphate pathway. Furthermore, there appears to be no shift to this alternate metabolic route prior to or during myelin formation, or during the later stage of myelin maintenance and neuronal maturation. The equal incorporation of specifically labelled glucose into phospholipids further substantiates these conclusions. The higher rate of metabolism of this material than of the more conventional tissue slices makes brain tissue developing in culture a most promising material for neurochemical studies.