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FURTHER STUDIES OF THE TRANSPORT OF PROTEIN TO NERVE ENDINGS *
Author(s) -
Barondes S. H.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb11619.x
Subject(s) - free nerve ending , leucine , axoplasmic transport , synaptic vesicle , protein biosynthesis , biochemistry , chemistry , nerve cells , microbiology and biotechnology , biology , vesicle , endocrinology , amino acid , membrane
Mice were injected intracerebrally with [l‐ 14 C]leucine, and the specific activities of subcellular fractions of brain and effractions of isolated nerve endings were determined. There was a progressive increase in the specific activity of protein associated with isolated nerve endings after incorporation of [l‐ 14 C]leucine into whole brain protein had terminated. Although, the incorporation of [ 14 C]leucine into soluble protein of whole brain did not differ significantly in mice which were 3 months or 1‐year old, the subsequent increase in specific activity of soluble protein isolated from nerve endings was significantly greater in the younger animals; 6‐month‐old mice were intermediate. Therefore, changes in some aspect of the transport of protein to nerve endings is altered even after sexual maturity. Anaesthetization with pentobarbitone during incorporation of [ 14 C]leucine into protein, and inhibition of protein synthesis with acetoxycycloheximide after incorporation of [ 14 C]leucine was complete, did not interfere with the subsequent appearance of radioactive protein at the nerve ending. Evidence is presented for the transport, from a proximal site of synthesis, of protein associated with particulate components of the nerve ending, including synaptic vesicles.