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HEXOSE TRANSLOCATION ACROSS THE BLOOD‐BRAIN INTERFACE: CONFIGURATIONAL ASPECTS 1
Author(s) -
Bidder T. George
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb10333.x
Subject(s) - chromosomal translocation , hexose , chemistry , biophysics , biology , biochemistry , gene , enzyme
— The translocation of intravenously administered tritiated 3‐ O ‐methyl‐ d ‐glucopyranose (3‐OMG) into brain has been studied in an intact nephrectomized rat preparation by infusing a series of hexoses into one internal carotid artery. 3‐OMG rapidly penetrated into brain with peak concentrations, attained 5–8 min after injection, declining to relatively constant values thereafter. 3‐OMG penetration exhibited saturation characteristics as shown by a levelling‐off in the rate of translocation as its calculated blood concentration was elevated above 50 m‐moles per 1000 ml. Infusion of d ‐galactose, d ‐glucose, 2‐deoxyglucose, d ‐xylose and d ‐mannose significantly reduced the translocation of 3‐OMG into brain on the side on which each was infused. d ‐glucosamine, d ‐mannitol and d ‐arabinose were without effect. The phenomenon of countertransport was demonstrated in the nephrectomized rat given tritiated 3‐OMG 20 hr prior to infusion of d ‐glucose or d ‐mannose into one carotid artery. Each of these hexoses reduced brain 3‐OMG concentration on the side on which it was infused. These results support the hypothesis that the translocation of certain hexoses across the blood‐brain interface is a carrier‐mediated process.