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CORRELATION BETWEEN [U‐ 14 C]GLUCOSE METABOLISM AND FUNCTION IN PERFUSED CAT BRAIN
Author(s) -
Otsuki S.,
Watanabe S.,
Ninomiya K.,
Hoaki T.,
Okumura N.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb10332.x
Subject(s) - glutamic acid , glutamine , aspartic acid , lactic acid , metabolism , citric acid cycle , amino acid , biochemistry , carbohydrate , glutamate receptor , chemistry , cats , medicine , tricarboxylic acid , carbohydrate metabolism , pyruvic acid , endocrinology , biology , bacteria , receptor , genetics
— In brain perfusion experiments conducted with blood containing [U‐ 14 C]glucose the relative specific activity (RSA) of blood glucose carbon incorporated in brain intermediate metabolites was measured. It was demonstrated that the so‐called metabolic pattern of G eiger is not constant, but it bears a close relation to the function of the brain. The results of the study may be summarized briefly as follows. (1) In a group (A) of cats with a high level of brain function, the RSA of lactic acid was 75 per cent; that of glutamic acid 80 per cent; aspartic acid 75 per cent; glutamine 61 per cent; GABA 43 per cent; and respiratory CO 2 55 per cent. It was observed that the major part of the carbon of amino acids, such as glutamic acid and aspartic acid, which are directly associated with the tricarboxylic acid cycle are derived from blood glucose. (2) In a group (B) showing a low level of brain function, the RSA of each amino acid was considerably lowered. The RSA of glutamic acid and aspartic acid was about 50 per cent and that of respiratory CO 2 was 27 per cent. (3) In a group (C) with a still lower level of brain function, each amino acid as well as the respiratory CO 2 had still lower RSA values. (4) The metabolic pattern of G eiger corresponds to values obtained during low functional activity of the brain in our experiment.