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SHORT CHAIN FATTY ACID INHIBITION OF RAT BRAIN Na‐K ADENOSINE TRIPHOSPHATASEI
Author(s) -
Dahl D. R.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb10327.x
Subject(s) - fatty acid , chemistry , enzyme , potassium , sodium , biochemistry , membrane , in vivo , molecule , in vitro , adenosine , stereochemistry , biology , organic chemistry , microbiology and biotechnology
— The possibility was considered that the sleep‐like state seen after injection of short chain fatty acids salts into animals is a result of inhibition of the sodium‐potassium activated ATPase. Tris salts of short chain fatty acids inhibited brain Na‐K ATPase activity in vitro at concentrations similar to intravenous levels causing narcosis in vivo. The inhibition depended on the logarithm of the concentration of a given acid. The concentration of acid anion which caused 50 per cent inhibition of the enzyme system (I 50 ) was determined for straight and branched chain acids with 4–12 carbon atoms per molecule. The log of I 50 concentrations plotted against the number of carbon atoms in the molecule gave a straight line; the inhibitory capacity of an acid increased by a factor of 2·3 for each ‐CH 2 ‐ added to the carbon chain.