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IN VITRO RNA SYNTHESES BY INTACT RAT BRAIN NUCLEI 1
Author(s) -
Dutton G. R.,
Mahler H. R.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb10321.x
Subject(s) - rna , in vitro , spermidine , ionic strength , in vivo , dna , biochemistry , biophysics , chemistry , tritium , biology , enzyme , genetics , gene , organic chemistry , aqueous solution , physics , nuclear physics
— The characteristics of DNA‐dependent RNA polymerase activity in intact rat cerebral cortex nuclei tested at low ionic strength are presented. The system was most dependent on the presence of spermidine and an ATP‐generating system and to a lesser extent on Mg 2+ and K + for maximal incorporation. Substitution of Mg 2+ by Mn 2+ or of K + by Na + resulted in substantially less activity than under the optimum conditions described. Maximal incorporation was about 10 per cent that of brain nuclear systems of high ionic strength. In addition, the labelling patterns of the in vitro RNA products were shown to be very similar to those found in vivo. The stability of isolated nuclei toward degradation of RNA synthetic capacity and products formed was much greater than that of a similar liver system.