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STUDIES ON SODIUM TRANSPORT IN RAT BRAIN NERVE‐ENDING PARTICLES
Author(s) -
Ling C.M.,
AbdelLatif A. A.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb10316.x
Subject(s) - ouabain , ficoll , chemistry , sodium , biophysics , sodium cyanide , isotopes of sodium , membrane , ultracentrifuge , cyanide , biochemistry , inorganic chemistry , biology , organic chemistry , peripheral blood mononuclear cell , in vitro
— (1) Nerve‐ending particles isolated from crude mitochondrial preparations from rat brain by discontinuous Ficoll density gradient ultracentrifugation were shown to possess a Mg 2+ and energy‐dependent transport system for Na + . (2) Ouabain or iodoacetate plus cyanide exerted an inhibitory effect on the outfiux but not the influx of Na + . (3) When K + was added to a medium containing particles loaded with Na + ( 22 Na), an immediate release of Na + from these particles was observed; this suggests the existence of a Na + –K + exchange transport system. (4) The K + effect was inhibited by 10 ‐4 M‐ouabain only at low (about 3·3 m m ), but not at high (20 m m ), K + concentrations. (5) The uptake and release of Na + by the nerve‐ending particles were found to be temperature‐dependent. (6) Only nerve‐ending particles with intact synaptosomal membranes were found to transport Na + actively.