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RATE OF PROTEIN RENEWAL IN SPINAL MOTONEURONS OF ADOLESCENT AND OLD RATS
Author(s) -
Jakoubek B.,
Gutmann E.,
Fischer J.,
Babický A.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb08962.x
Subject(s) - turnover , protein turnover , spinal cord , biology , component (thermodynamics) , medicine , endocrinology , chemistry , protein biosynthesis , neuroscience , biochemistry , physics , management , economics , thermodynamics
— The rate of incorporation of [ 35 S]methionine and the turnover rate of proteins in spinal motoneurons were studied in adolescent and old rats. The radioactivity of proteins was estimated by quantitative autoradiography and direct counting of beta radiation from samples of neurons isolated by free hand dissection. Both methods showed that the rate of incorporation into spinal motoneurons was significantly lower in old animals. By measuring the turnover rate of proteins in spinal motoneurons at least two protein components could be distinguished by their different turnover rates. The short‐lived component had an average half‐life of 2‐2‐2‐8 days, the long‐lived protein component had an average half‐life of 27‐38 days. Neither in the short‐lived component, nor in the long‐lived component was it possible to detect a significant difference between adolescent and old rats.