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STUDIES OF WATER SOLUBLE LIPOPROTEINS IN RAT BRAIN *
Author(s) -
Herschkowitz N.,
Mckhann† G. M.,
Shooter E. M.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb06191.x
Subject(s) - acrylamide , lipoprotein , polyacrylamide gel electrophoresis , electrophoresis , staining , gel electrophoresis , chromatography , chemistry , gel electrophoresis of proteins , biochemistry , two dimensional gel electrophoresis , microbiology and biotechnology , cholesterol , biology , enzyme , proteomics , copolymer , organic chemistry , gene , genetics , polymer
—The occurrencc of water soluble lipoprotein in the 105,000 g supernatant of rat brain homogenate has been demonstrated. The lipoproteins of brain differed from those of the serum with respect to lipid composition, density and reaction to serum lipoprotein antibodies. Acrylamide gel electrophoresis resolved the brain supernatant into four zones which stained with lipid dye. It was possible to demonstrate that these zones also contained protein by double staining of the gel for lipid and protein. Two of the zones differed in their mobility from serum lipoprotein. Preparative acrylamide gel electrophoresis isolated protein fractions in supematant which contained both lipid and protein and permitted preliminary identification of the lipoprotein bands amongst all the protein bands resolved by acrylamide gel electrophoresis.