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ACID PHOSPHATASE LOCALIZATION IN INDIVIDUAL NEURONS BY A QUANTITATIVE HISTOCHEMICAL METHOD *
Author(s) -
Hirsch Hilde E.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb06183.x
Subject(s) - acid phosphatase , phosphatase , neuroscience , chemistry , biochemistry , computational biology , biology , enzyme
—By an adaptation of the fluorometric method of C ampbell and Moss (1961), the activity of α‐naphthyl acid phosphatase was measured in individual neurons of monkey and human spinal cord and found to be many times higher in nerve cell bodies than in the surrounding neuropil. It was also measured in cerebellar cortex and found most concentrated in the granular (neuronal) layer. As this distribution is distinctive and paralleled by two other acid hydrolases, β‐galactosidase and β‐glucuronidase, it is considered to offer additional support for the lysosomal concept in nervous tissue and to indicate that nerve cell perikarya are much richer in lysosomes than are axons, dendrites or glial cells.