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IDENTIFICATION OF MULTIPLE FORMS OF AMINOBUTYRATE TRANSAMINASE IN MOUSE AND RAT BRAIN : SUBCELLULAR LOCALIZATION
Author(s) -
Waksman A.,
Bloch M.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb06180.x
Subject(s) - isozyme , transaminase , biochemistry , electrophoresis , biology , immunoelectrophoresis , identification (biology) , gaba transaminase , enzyme , chemistry , microbiology and biotechnology , genetics , antibody , glutamate decarboxylase , botany
—(1) At least four distinct molecular forms of 4‐aminobutyrate: 2‐oxoglutarate aminotransferase from mouse and rat brain, have been separated by electrophoresis on paper, cellogel, agargel, silicagel and by immunoelectrophoresis. (2) The existence of specific typical electrophoretic profiles in mitochondrial and extramitochondrial compartments was shown. (3) A differential effect of pH on the anionic and cationic 4‐aminobutyrate:2‐oxoglutarate aminotransferase transaminase activities has been shown. (4) The possible consequences of the 4‐aminobutyrate: 2‐oxoglutarate aminotransferase isozyme compartimentation on the local availability of γ‐aminobutyric acid pools has been discussed.