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PYRIMIDINE SYNTHESIS IN TISSUE CULTURE 1
Author(s) -
Appel S. H.,
Silberberg D. H.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb05925.x
Subject(s) - orotic acid , pyrimidine , biochemistry , carbamyl phosphate , pyrimidine metabolism , perchloric acid , uridine , chemistry , rna , enzyme , organic chemistry , purine , gene
— Myelinated cerebellar tissue culture (organ culture) was used to assess the salvage and de novo pathways of pyrimidine synthesis in mammalian brain. Radioactive orotic acid and carbamyl aspartic acid were readily incorporated into UMP and into perchloric acid‐insoluble RNA. The incorporation was effectively blocked by azauridine. Neither radioactive sodium bicarbonate or citrulline was incorporated into UMP or blocked by azauridine. [ 3 H]Uridine, on the other hand, rapidly entered the cultures, was incorporated into UMP and perchloric acid‐insoluble material, and was partially inhibited by azauridine. The failure to demonstrate activity of carbamyl phosphate synthetase suggests the potential importance of the salvage pathway and the likely dependence of the brain upon exogenous and endogenous pyrimidine precursors.