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BRAIN CARDIOLIPIN: ISOLATION AND FATTY ACID POSITIONS 1
Author(s) -
Yabuuchi H.,
O'Brien J. S.
Publication year - 1968
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1968.tb05920.x
Subject(s) - cardiolipin , silicic acid , chemistry , chromatography , fatty acid , column chromatography , phospholipid , biochemistry , glycerol , hydrolysis , membrane , organic chemistry
— Cardiolipin (diphosphatidyl glycerol) was isolated from bovine brain grey matter in pure form by column chromatography. A combination of a DEAE cellulose column, an acid‐silicic acid column and a bicarbonate‐treated silicic acid column was used for the isolation. Analytical data on cardiolipin (and lyso‐cardiolipin), including phosphorus and fatty ester values, infrared and ultraviolet spectra, and chromatographic behaviour of intact cardiolipin, lyso‐cardiolipin and their water‐soluble hydrolysis products gave results which were consistent with the diphosphatidylglycerol structure proposed by MacFarlane. Cardiolipin constituted 1·2 per cent of the total lipids from grey matter, or 0·43 per cent of the dry weight. No aldehydes were detected in purified cardiolipin. The major fatty acids were 16:0, 16:1, 18:1, 18:2 and 20:4. Cardiolipin contained much higher concentrations of 18:2 (17·8 per cent) than any of the other major grey matter glycerophosphatides. Cardiolipin was hydrolysed by incubation with snake venom ( Trimeresurus flavoviridis ) phospholipase A (phosphatide acyl‐hydrolase, EC 3.1.1.4) to determine the distribution of fatty acids on the carbons of its diglyceride moieties. The fatty acids were not distributed randomly; 16:0, 18:0 and 18:2 were predominantly localized in the α and α positions, while 18:1 and 20:4 were predominantly localized in the β and β positions.