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OCCURRENCE OF N ‐ACETYL‐ l ‐GLUTAMIC ACID IN THE HUMAN BRAIN
Author(s) -
Auditore Joseph V.,
Wade Littleton,
Olson Erik J.
Publication year - 1966
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1966.tb04272.x
Subject(s) - glutamic acid , ninhydrin , chemistry , hydrolysis , acid hydrolysis , hydrazide , reagent , chromatography , ion chromatography , biochemistry , organic chemistry , amino acid
SUMMARY A substance apparently identical with N ‐acetyl‐ l ‐glutamic acid was isolated from an aqueous extract from human brain by a combination of paper and ion exchange chromatography. The isolated substance does not react with ninhydrin reagent but yields glutamic acid upon acid hydrolysis. Acetyl hydrazide was identified by paper chromatography of hydrazinolysates of the isolated substance and N ‐acetyl‐ l ‐glutamic acid. The configuration was determined with l ‐specific hog kidney acylase.