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SOME OBSERVATIONS ON THE ESTIMATION OF TRYPTAMINE IN TISSUES
Author(s) -
Eccleston D.,
Ashcroft G. W.,
Crawford T. B. B.,
Loose R.
Publication year - 1966
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1966.tb03336.x
Subject(s) - tryptamine , tryptophan , amine gas treating , chemistry , chromatography , ion chromatography , biogenic amine , serotonin , biochemistry , organic chemistry , amino acid , receptor
SUMMARY The method of H ess and U denfriend (1959) for the estimation of tryptamine in tissues, is demonstrated to allow the carry over of tryptophan to the final extract, resulting in falsely high values for the amine. A modification of the original method is described, incorporating ion‐exchange chromatography, which gives complete separation of these components. Using this method, tryptamine is found neither in the brain of normal guinea pigs, nor in animals treated with l ‐tryptophan. It is found only when l ‐tryptophan is administered following pretreatment with an amine oxidase inhibitor.