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Genetic variants of interleukin‐1RN and interleukin‐1β genes and risk of cervical cancer
Author(s) -
Singh H,
Sachan R,
Goel H,
Mittal B
Publication year - 2008
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.2007.01655.x
Subject(s) - gene , interleukin , cancer , cervical cancer , interleukin 1β , genetics , medicine , biology , immunology , cytokine
Objectives Inflammation plays a major role in pathogenesis of cervical cancer. We planned to study whether polymorphisms in inflammation‐related genes, IL‐1RN (VNTR) and IL‐1β (‐511C/T), are associated with risk of cervical cancer. Design Case–control study. Setting Uttar Pradesh state in India. Sample One hundred and fifty, histopathologically confirmed cases with cervical cancer and 162 age‐, ethnicity‐matched, cervical cytology negative, healthy controls were recruited to this study. Methods Genotyping of IL‐1RN (VNTR) and IL‐1β (‐511C/T) polymorphisms was performed using polymerase chain reaction (PCR)/PCR–restriction fragment length polymorphism. Power of study was 80% with type 1 error of 0.05. Haplotypes frequencies were obtained by computer package ‘Arlequin’. Main outcome measures Haplotype IL‐1RN *2/ IL‐1β *T is associated with higher risk and of cervical cancer. Results IL‐1RN genotypes 1/2 and 2/2 were associated with significantly elevated risk of cervical cancer (OR = 3.3; P = 4.9 × 10 −6 and OR = 2.9, P = 0.02). Similarly, TT genotype of IL‐1β polymorphism was significantly higher in cases compared with controls (57.7 versus 38.3%; OR = 2.8; P = 0.012). 2/2 genotype of IL‐1RN (OR = 4.8, P = 0.0006) and TT genotype of IL‐1β (OR = 5.2; P = 0.02) were associated with the higher stages (III) of cervical cancer. Haplotypes 1T ( IL‐1RN *1/ IL‐1β *T) and 2T ( IL‐1RN *2/ IL‐1β *T) were also significantly associated with higher susceptibility to cervical cancer and its progression. Logistic regression analysis suggests IL‐1RN allele 2 and IL‐1β ‐511T were independently associated with increased risk for cervical cancer. Conclusion IL‐1RN* 2 and IL‐1β ‐511* T in various combinations of genotypes and haplotypes are associated with higher susceptibility for cervical cancer.