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Thiol status and antioxidant capacity in women with a history of severe pre‐eclampsia
Author(s) -
Raijmakers Maarten T.M.,
Roes Eva Maria,
Zusterzeel Petra L.M.,
Steegers Eric A.P.,
Peters Wilbert H.M.
Publication year - 2004
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.2004.00051.x
Subject(s) - homocysteine , eclampsia , antioxidant , oxidative stress , antioxidant capacity , medicine , glutathione , thiol , pregnancy , endocrinology , family history , plasma homocysteine , chemistry , biochemistry , enzyme , biology , genetics
Objective  To investigate a possible mechanism that could lead to the subsequent development of cardiovascular diseases (CVD) in women with a history of severe pre‐eclampsia. Design  Case–control study. Setting  University Medical Centre Nijmegen, The Netherlands. Sample  Non‐pregnant women with a history of severe pre‐eclampsia ( n = 131) and women with an uncomplicated obstetric history ( n = 94). Methods  Total plasma levels of cysteine (tCys), homocysteine (tHcy), cysteinylglycine (tCysGly) and glutathione (tGSH), the free‐to‐oxidised ratio of these thiols in whole blood, the glucose‐6‐phosphate dehydrogenase (G6PDH) enzyme activity and antioxidant capacity were assessed at least 6 months following last pregnancy. Main outcome measure  Oxidative stress and antioxidant status. Results  Women with a history of severe pre‐eclampsia showed higher levels (mean [SD]) of tHcy (13.1 [5.0] versus 11.5 [4.8] μmol/L; P = 0.018) and tCysGly (37.5 [5.6] versus 34.0 [5.8] μmol/L; P = 0.0001) compared with controls, whereas tCys was lower (232 [31] versus 242 [39]; P = 0.029). The lower free‐to‐oxidised ratio of homocysteine (2.3 [0.8] versus 2.9 [1.0], P = 0.0001) among women with a history of severe pre‐eclampsia as compared with control subjects might indicate a higher oxidant status for homocysteine. Previous severe pre‐eclamptic patients had also a higher antioxidant capacity as compared with controls (0.79 [0.14] versus 0.74 [0.11] mmol Fe 2+ /L, P = 0.002). Conclusion  Since women with a history of severe pre‐eclampsia showed elevated total homocysteine levels, which is an independent risk factor for CVD, and higher oxidised homocysteine levels in whole blood, these women may have an enhanced risk for the subsequent development of cardiovascular‐related problems in later life.

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