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A novel in vitro co‐culture system for the study of maternal decidual endothelial cell–trophoblast interactions in human pregnancy
Author(s) -
Gallery Eileen D.M.,
Campbell Suzanne,
Ilkovski Biljana,
Sinosich Michael J.,
Jackson Christopher
Publication year - 2001
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.2001.00127.x
Subject(s) - cytotrophoblast , trophoblast , placentation , decidua , in vitro , microbiology and biotechnology , placenta , decidual cells , biology , endothelial stem cell , pregnancy , in vivo , andrology , medicine , fetus , genetics
Investigation of the pathophysiology of pre‐eclampsia (characterised by insufficient invasion of the intrauterine vasculature by cytotrophoblasts) has been hampered by the absence of a suitable animal model, and ethical constraints in clinical studies. We have developed a novel in vitro human cell co‐culture system allowing direct assessment of cytotrophoblast invasion of a decidual endothelial cell monolayer from the abluminal side, as occurs in vivo . This model will facilitate detection, at the cellular level, of abnormal endothelial cell–trophoblast functional interactions in pre‐eclampsia and other pregnancy disorders with abnormal placentation.