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Roles of prostaglandins and intracellular free calcium mobilisation in epidermal growth factor‐induced proliferation of human amnion cells
Author(s) -
Tahara Masahiro,
Masumoto Nobuyuki,
Mizuki Jirou,
Kasahara Kanji,
Tasaka Keiichi,
Kurachi Hirohisa,
Miyake Akira
Publication year - 1994
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.1994.tb13551.x
Subject(s) - intracellular , egta , epidermal growth factor , calcium in biology , dna synthesis , amnion , endocrinology , medicine , cell growth , prostaglandin e , biology , cell culture , calcium , chemistry , microbiology and biotechnology , biochemistry , in vitro , fetus , genetics , pregnancy
Objective To investigate the mechanisms which regulate the growth of human amnion cells. Design A prospective descriptive study. Subject Women undergoing caesarean section at term before the onset of labour. Interventions Amnion cells were cultured in monolayer. Main outcome measures Cell cycle analysis, intracellular calcium levels, prostaglandin (PG) production rates. Results Epidermal growth factor (EGF) stimulated intracellular Ca 2+ mobilisation and PGE 2 production in cultured amnion cells. The addition of a Ca 2+ channel blocker (cobalt) or a Ca 2+ chelator (EGTA) into the culture medium inhibited intracellular Ca 2+ mobilisation and PGE 2 production induced by EGF. The analysis of cell cycles showed that EGF induced the initiation of DNA synthesis and that the addition of cobalt or EGTA into the culture medium inhibited EGF‐induced DNA synthesis. The addition of a cyclo‐oxygenase inhibitor (indomethacin) inhibited PGE 2 production and DNA synthesis induced by EGF without the effect on intracellular Ca 2+ mobilisation. Moreover, the inhibitory effect of indomethacin on EGF‐induced DNA synthesis was attenuated by the addition of exogenous PGE 2 or PGF 2n . Conclusions These data suggest that EGF induces an increase in intracellular Ca 2+ levels and the rate of prostaglandin production which leads to proliferation of human amnion cells.