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The immunoperoxidase localization of tumour markers in ovarian cancer: the value of CEA, EMA, cytokeratin and DD9
Author(s) -
HAMMOND R. H.,
BATES THELMA D.,
CLARKE D. G.,
GRANT ANNE G.,
HAINES A. M. R.,
EUSTACE D. L. S.,
HEYDERMAN EADIE
Publication year - 1991
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.1991.tb10315.x
Subject(s) - carcinoembryonic antigen , immunoperoxidase , cytokeratin , pathology , ovarian cancer , immunoscintigraphy , immunohistochemistry , colonic cancer , medicine , antigen , colonic carcinoma , staining , colorectal cancer , cancer , carcinoma , antibody , monoclonal antibody , immunology , radioimmunotherapy
Summary. Primary tumours from 40 patients with epithelial ovarian cancer, treated at St Thomas's Hospital over a 10‐year period, were studied for the immunocytochemical expression of the following tumour markers in formalin‐fixed paraffin embedded material: carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), cytokeratin (CAM 5.2), and DD9. An indirect immunoperoxidase staining technique was used. All of the tumours were positive for EMA and CAM 5.2, and 30% of them were positive for both CEA and DD9. The absence of CEA and DD9 may be of value in differentiating between metastatic abdominal adenocarcinomas of ovarian origin and those of gastrointestinal origin, but no indication of prognosis was obtained using these epithelial markers. The strong and widespread staining of all of the tumours for EMA suggests that this may be a useful marker for detecting metastatic or recurrent disease by immunoscintigraphy.