Premium
A comparison of fixed regimens for obtaining human cleaving oocytes for research purposes
Author(s) -
MESSINIS IOANNIS E.,
TEMPLETON ALLAN,
ANGELL ROSLYN,
AITKEN JOHN
Publication year - 1986
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.1986.tb07811.x
Subject(s) - andrology , regimen , follicular phase , in vitro fertilisation , human fertilization , cleavage (geology) , follicular fluid , stimulation , oocyte , gynecology , medicine , biology , embryo , anatomy , paleontology , fracture (geology) , microbiology and biotechnology
Summary. A fixed schedule for ovarian stimulation and follicular aspiration, previously used in our department for research purposes, was modified in an attempt to increase the recovery and cleavage rates of the oocytes. Three different clomiphene regimens were used to stimulate the ovaries of normal volunteer women requesting laparoscopic sterilization (50 mg and 150 mg daily for 5 days, and 50 mg daily for 10 days). Oocytes were recovered from 83% of the aspirated follicles, i.e. 1·6 oocytes/patient: 65% of the oocytes cleaved after in‐vitro fertilization and, on average, 1·0 cleaving egg was obtained per patient. There were no differences in the recovery and cleavage rates between the three clomiphene regimens. It is concluded that a sufficient number of cleaving embryos for research purposes can be generated with the present fixed regimen, which offers little inconvenience to the volunteers as the day of egg recovery can be predicted some time in advance.