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Monoclonal antibodies for the histopathological diagnosis of cervical neoplasia
Author(s) -
JHA R. S.,
WICKENDEN C.,
ANDERSON M. C.,
COLEMAN D. V.
Publication year - 1984
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.1984.tb04788.x
Subject(s) - pathology , epithelium , monoclonal antibody , antibody , cervical intraepithelial neoplasia , immunohistochemistry , staining , neoplastic transformation , malignant transformation , biology , intraepithelial neoplasia , medicine , cervical cancer , cancer , immunology , carcinogenesis , prostate , genetics
summary Five monoclonal antibodies (Cal, HMFG 1 and 2, 8.30.3 and 77.1) were used to study the distribution of antibody binding sites in cervical tissue with a view to identifying a marker which would distinguish between benign and malignant cervical epithelium. Both benign tissue (mature and immature metaplastic squamous epithelium, congenital transformation zone and glandular epithelium) and neoplastic tissue (cervical intraepithelial neoplasia, 1, 2 and 3 and invasive squamous cell carcinoma) were stained by these antibodies. Although immature metaplastic epithelium stained strongly with all the antibodies, the intensity and distribution of staining in general did not distinguish between benign and neoplastic conditions. All five antibodies, raised against three different antigens, stained cervical tissue in a similar way and thus were unsuitable for use as specific tumour markers in equivocal cases. Further studies on other tumour markers are indicated in view of the potential value of this aroach.