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THE EFFECTS OF HYPOXIA ON HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN PLACENTAL VILLI MAINTAINED IN ORGAN CULTURE
Author(s) -
Sharp Frank,
Carty MatthewJ.,
Young Hamish
Publication year - 1972
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.1972.tb15747.x
Subject(s) - hypoxia (environmental) , hydroxysteroid dehydrogenase , placenta , dehydrogenase , endocrinology , medicine , hydroxysteroid dehydrogenases , organ culture , enzyme , enzyme assay , biology , chemistry , fetus , andrology , biochemistry , oxygen , in vitro , pregnancy , genetics , organic chemistry
Summary Hydroxysteroid dehydrogenase (HSD) enzyme activity has been studied histochemically in placental villi maintained in organ culture, some under fully oxygenated conditions and some under conditions of hypoxia. HSD activity was diminished in hypoxia. The process is apparently selective, with trophoblastic activity more sensitive than that in villous core, and 3β‐HSD more so than 17β‐HSD. Also, with 17β‐HSDs, activity using oestradiol‐17β as substrate was more sensitive than that using testosterone as substrate. The possible correlation with the clinical picture in placental insufficiency is discussed.