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PREPARATION OF CERVICAL SCRAPE MATERIAL FOR AUTOMATIC SCREENING
Author(s) -
Tucker J. H.,
Gresham G. A.
Publication year - 1971
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/j.1471-0528.1971.tb00210.x
Subject(s) - syringe , haematoxylin , suspension (topology) , eosin , hypodermic needle , materials science , suspension culture , balanced salt solution , biomedical engineering , chromatography , chemistry , staining , pathology , medicine , biology , mathematics , cell culture , organic chemistry , psychiatry , homotopy , pure mathematics , genetics
Summary A method of preparing cervical scrape specimens for automatic analysis is described. A cell suspension is obtained by washing the spatula used to scrape the cervix in a balanced salt solution. Cell clumps are disaggregated by pumping the specimen to and fro through a hypodermic syringe needle, and the resulting suspension is centrifuged, fixed and pipetted onto a glass slide. Specimens are stained with haematoxylin and eosin.