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Characterisation of a cell‐free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
Author(s) -
DE LAS M CARDOSO MARÍA,
MANZO RICARDO M,
TONARELLI GEORGINA G,
SIMONETTA ARTURO C
Publication year - 2012
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.2012.00852.x
Subject(s) - enterococcus faecalis , antimicrobial , microbiology and biotechnology , bacteria , strain (injury) , agar , antibacterial activity , escherichia coli , chemistry , pathogenic bacteria , agar plate , biology , catalase , food science , enzyme , staphylococcus aureus , biochemistry , gene , anatomy , genetics
Enterococcus faecalis DBFIQ E24 strain produces antimicrobial substances that inhibit the growth of food‐borne pathogenic bacteria, yeasts and moulds. This broad antimicrobial spectrum was determined by obtaining the cell‐free supernatant (CFS) and employing the agar‐well‐diffusion method. Its antibacterial activity was completely inactivated by the action of proteinase K and not affected by catalase action. Besides, it was stable to thermal treatments, showing maximum antibacterial activity at pH 7.0. Moreover, CFS showed a bactericidal effect against Escherichia coli DBFIQ Ec9 strain. All these interesting physicochemical properties allow that either CFS or E. faecalis DBFIQ E24 strain to be considered as potential food biopreservatives.