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Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines
Author(s) -
KIM YOUNGHOON,
KIM MAE JIN,
HAN KYOUNG SIK,
IMM JEE YOUNG,
OH SEJONG,
KIM SAE HUN
Publication year - 2009
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.2009.00466.x
Subject(s) - lactoferrin , colostrum , chemistry , hela , cancer , sephadex , affinity chromatography , cell culture , cancer cell , cell , cervix , microbiology and biotechnology , biology , immunology , biochemistry , medicine , antibody , genetics , enzyme
In the present study, lactoferrin was purified from caprine colostrum and its anticancer effects were investigated on various cancer cell lines, including lung, colon, cervix, stomach and breast cancer cells. The caprine lactoferrin (CLF) was purified by ultrafiltration and sequential chromatography using a CM‐Sephadex C‐50‐120 ion‐exchange column and affinity chromatography on Hi‐Trap TM Heparin HP. The purified CLF exhibited antiproliferative effects on the five tested cancer cell lines in a dose‐dependent manner, with 20–30% survival as compared to the control. Interestingly, the most intense inhibition of cell growth was observed on the ZR‐75‐1 breast cancer cells (IC 50 = 27.5 µg/mL). Our findings suggest, for the first time, that purified CLF from caprine colostrum may constitute a novel anticancer agent for the food industry.