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The acid tolerance association with expression of H + ‐ATPase in Lactobacillus casei
Author(s) -
CHEN XIA,
SUN ZHIHONG,
MENG HE,
ZHANG HEPING
Publication year - 2009
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.2009.00461.x
Subject(s) - lactobacillus casei , probiotic , lactic acid , atpase , biochemistry , bacteria , lactobacillus , chemistry , enzyme , biology , fermentation , genetics
Acid and bile salt tolerance are considered as important properties for lactic acid bacteria to act as probiotics. Moreover, H + ‐ATPase is acting for the production of ATP to the transmembrane proton motive force and can either generate ATP or generate a PMF using ATP produced by fermentative substrate‐level phosphorylation. In this work, we isolated a potential probiotic organism from home‐made koumiss and detected the expression of H + ‐ATPase in distinct acid conditions using the reverse transcription polymerase chain reaction method. The results show that Lactobacillus casei Zhang can be considered as potentially probiotic to some extent: and that the expression of H + ‐ATPase is associated with the acidity of the environment in L. casei.