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Factors affecting the adsorption of Lactobacillus plantarum bacteriocin bacST8KF to Enterococcus faecalis and Listeria innocua
Author(s) -
TODOROV SVETOSLAV D,
POWELL JILLIAN E,
MEINCKEN MARTINA,
WITTHUHN R CORLI,
DICKS LEON M T
Publication year - 2007
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/j.1471-0307.2007.00327.x
Subject(s) - lactobacillus sakei , bacteriocin , enterococcus faecalis , listeria , lactobacillus plantarum , lactobacillus casei , chemistry , lactobacillus salivarius , microbiology and biotechnology , lactobacillus , food science , listeria monocytogenes , lactic acid , bacteria , biology , antimicrobial , biochemistry , escherichia coli , fermentation , genetics , gene
Bacteriocin ST8KF (bacST8KF), produced by Lactobacillus plantarum ST8KF isolated from kefir, inhibits the growth of Enterococcus faecalis E88, Lactobacillus casei LHS, Lactobacillus curvatus DF38, Lactobacillus sakei DSM 20017 T , Lactobacillus salivarius 241 and Listeria innocua LMG 13568. Eighty per cent of bacST8KF adsorbed to La. casei LHS and La. sakei DSM 20017 T . However, the same level of adsorption was recorded to bacST8KF‐resistant Lactobacillus paraplantarum ATCC 700211 T and Streptococcus caprinus ATCC 700066, suggesting that adherence to the cell surface occurs at random and not always at specific receptor sites. Adsorption of bacST8KF is affected by inorganic salts, acids, surfactants, alcohols, temperature and pH, as observed for E. faecalis E88 and Li. innocua LMG 13568. The target of bacST8KF is the cytoplasmic membrane, as indicated by changes in cell morphology and leakage of DNA and β‐galactosidase from cells of E. faecalis E88 and Li. innocua LMG 13568 treated with bacST8KF. However, the mode of activity against E. faecalis E88 and Li. innocua LMG 13568 is bacteriostatic, as concluded from slight increases in optical density levels after a few hours in the presence of bacST8KF. Activity of bacST8KF doubled (from 12 800 AU mL −1 to 25 600 AU mL −1 ) when strain ST8KF was grown in the presence of Li. innocua LMG 13568, which may indicate some sort of quorum sensing.